Comparative analysis of different methods of DNA extraction from human breast tumor samples

Abstract

Introduction: A large portion of researches conducted worldwide depend on stored samples that will most often be subjected to the process of extracting genetic material for molecular biology analysis. These samples archived are an extremely valuable source for retrospective genetic studies. These include mutation screening in cancer-critical genes as well as pathogen detection. Aim: The aimed of this study were to evaluate different methods of DNA extraction in frozen tissue, FFPE and blood.  Results: We compared 13 different DNA extraction methods. All 63 samples (20 FFPE tissues, 23 frozen tissues and 20 blood) were obtained from women undergoing breast cancer treatment at Barretos Cancer Hospital. The purity and yield of the extracted DNA were evaluated by NanoDrop® Spectophotometer. All extractions independently sample used were found suitable for amplification of fragments up 311 bp amplification was marginally successful. Conclusion: QIAamp DNA FFPE Tissue kit was found to have better quality for FFPE samples and also excellent yield. For frozen tissue samples, excellent purity and yield values were seen for QIAamp DNA Mini and Blood Mini Kit. Buffy coat samples showed better results with QIAamp DNA Mini Blood and Mini Kit. We conclude that the extraction method plays an important role regarding performance in molecular applications.