Modelo experimental de endometriose em ratas: caracterização e validação por meio de análise histológica e enzimática

Authors

  • Fabiana Albani Zambuzi
  • Maria Luiza Nunes Mamede Rosa
  • Andrea Carla Celotto

Keywords:

Endometriose, neutrófilo, inflamação, ratas, modelo experimental

Abstract

Introduction: Endometriosis is a disease characterized by the presence of endometrial tissue outside the uterine cavity, which affects a significant number of women of reproductive age and presents a complex and invasive diagnosis. Aim: Characterize and validate an experimental model of endometriosis described in the literature using Wistar rats. The study evaluated i) the ability to perform the endometriosis in rats and ensure the reproducibility of the model; ii) the feasibility of obtaining histopathological samples of the implants that allow characterizing the disease. Material and Methods: Endometriosis was induced in adult female rats that had a fragment of the uterine horn removed and implanted in the peritoneal cavity. After 21 days, the implants were extracted for histological analysis and metalloproteinase (MPO) measurement. Results: It was demonstrated that there was adherence of the implant on peritoneum and it also showed the formation of adhesion on intestinal loops and abdominal adipose tissue, cysts and new blood vessels. From the histological analysis, the characteristic development of the endometrium compatible with the estrus phase was observed, with the presence of cubic cells, evident nucleus and infiltration of neutrophils in the implants, in addition to MPO detection. Conclusion: The model used is easy to execute and has good reproducibility, capable of reproducing the morphological characteristics of endometriosis, in addition to making it possible to obtain samples to assess the functional changes of immune cells, as well as the detection of mediators produced by these cells which also are related to the endometriosis pathogenesis.

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Published

2020-12-30

How to Cite

Zambuzi, F. A., Rosa, M. L. N. M., & Celotto, A. C. (2020). Modelo experimental de endometriose em ratas: caracterização e validação por meio de análise histológica e enzimática. Manuscripta Medica, 3, 4–13. Retrieved from https://manuscriptamedica.com.br/revista/index.php/mm/article/view/48

Issue

Vol. 3 (2020): Manuscripta Medica

Section

Ciências Biológicas

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